منابع مشابه
Isolation of Rat Liver Plasma Membranes
Nucleotide pyrophosphatase and phosphodiesterase I of rat liver have been found to be localized primarily in cell particulates highly enriched with respect to the most commonly accepted plasma membrane marker, 5'-nucleotidase, and therefore should themselves be assigned a plasma membrane localization. The observation that plasma membranes sediment in isotonic sucrose with both nuclear and micro...
متن کاملPlasma Membranes of the Rat Liver
A method is described for the rapid isolation of a plasma membrane fraction containing a high concentration of intact bile canaliculi from the rat liver. Isolated bile canaliculi retain most of the ultrastructural features exhibited in the intact liver cell. The final fraction contains 5'-nucleotidase activity at approximately the same concentration as that in previous preparations of plasma me...
متن کاملISOLATION OF RAT LIVER PLASMA MEMBRANES Use of Nucleotide Pyrophosphatase and Phosphodiesterase I as Marker Enzymes
Nucleotide pyrophosphatase and phosphodiesterase I of rat liver have been found to be localized primarily in cell particulates highly enriched with respect to the most commonly accepted plasma membrane marker, 5'-nucleotidase, and therefore should themselves be assigned a plasma membrane localization. The observation that plasma membranes sediment in isotonic sucrose with both nuclear and micro...
متن کاملIsolation and partial characterization of a fatty acid binding protein in rat liver plasma membranes.
When [14C]oleate-bovine serum albumin complexes were incubated in vitro with rat liver plasma membranes (LPM), specific, saturable binding of oleate to the membranes was observed. Maximal heat-sensitive (i.e., specific) binding was 3.2 nmol/mg of membrane protein. Oleate-agarose affinity chromatography of Triton X-100-solubilized LPM was used to isolate a single 40-kDa protein with high affinit...
متن کاملSpecific binding of calcitonin to rat liver plasma membranes.
The binding of human calcitonin (CT) was investigated in the plasma membrane fraction obtained from normal rat liver. The liver plasma membrane bound 125I-labeled [( 125I]) human CT, with increasing concentrations of the plasma membrane protein from 4.6-80 micrograms/ml, in a both time and temperature dependent manner. Specific binding of [125I] human CT was competitively inhibited by concentra...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1970
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.47.3.604